Research & Development/Project

Cell Respiration Assay System:CRAS


CRAS Overview


Measurement
    ~Measuring by using electrochemical measurement technology


Non-Invasiveness
    ~Non-invasively measuring oxygen consumption (respiratory volume)
       of cell (fertilized egg)


Objective Assessment
    ~It is possible to objectively assay quality instead of morphological
       subjectively assay quality

Cell Respiration Assay System:CRAS is a fine assay system that utilizes electrochemical measurement technology.
This system is made up of potentiostat, inverted microscope, and internal respiratory measurement software PC.
By scanning a microprobe, it electrochemically measures the concentration gradient of dissolved enzyme in the offshore and proximity of the sample and calculates the respiratory volume with the spherical diffusion formula. The measuring plate used in this measurement has six inverted conical wells, which allows continuous measurements.
This device measures non-intrusively by installing microprobes by the sample. This measurement does not affect cell respiratory activity or embryogenesis.

Our product "CRAS-3.0" infomation (Japanese site)

 
Quality assessment through measurement

    ※Click on the image to enlarge


View of the measurement. The respiratory activity of one cell (one fertilized egg) can be measured by placing the microprobe by the sample that is placed at the center of the well and running the scanner up and down.
 

The respiratory volume in the mouse embryogenesis process is shown to increase along with the progress of the development.

※Click on the image to enlarge   ※Click on the image to enlarge
 

Respiratory volume of mouse blastocyst grown in each culture medium
Comparison of the cell count and respiratory volume of mouse blastocyst grown from two cellular embryos with different culture solutions
There is no difference in the cell count of the blastocysts between the medium cultures of Company A and Company B, but there is a difference in the respiratory volume.
The culture medium of Company C showed a result of low cell count and respiratory volume.
The measurement from this device is a measurement system that can evaluate the embryonic activities that cannot be observed. This device is also used in the embryonic transplantation of live stock.

Reference
Abe H and Hoshi H, J Reprod Dev. 2003 Jun;49(3):193-202
Abe H, J Mamm Ova Res. 2007 Dec;24(3):70-78
Shiku H et al.,Anal Chem. 2001 Aug 1;73(15):3751-8


Equipment

   
CME-0002
microprobe
  CMP-0001
measuring plate
  CRE-0001
referenceelectrode for measuring
※ Handle with care
This product is for research purpose and should not be used for in vitro diagnosis or clinical purpose.
Please understand that the specification may be changed without notice for upgrading.

Please feel free to contact us for any consultation/request, price inquiry, or test device rental.